Loss of Ang-2 results in attenuated intracellular calcium increase in response to VEGF and histamine <i>in vitro</i>.

Abstract

<p>Immortalized murine lung endothelial cells from WT and Ang-2^−/− mice (WT EOMA, KO EOMA) were loaded with Fluo-4 calcium sensitive dye and the relative fluorescence (stimulated/unstimulated fluorescence) was quantified in response to VEGF (25 ng/ml) and histamine (10⁻⁵M) after 10 min stimulation. HUVEC served as positive control (n = 3, **p<0.005 WT vs. KO).</p