<p>All panels show representative Western blot results after 5 rounds of sPMCA. Samples considered CWD positive are denoted with an asterisk (*). (A-H) Complete PK digestion of PrP<sup>C</sup> is shown in negative deer brain controls (-C) without and with PMCA. PrP<sup>CWD</sup> detection is shown in positive deer controls (+C) following PK digestion without and with PMCA. Tissue abbreviations are defined as follows: C = control, MED = mediastinal lymph node, PAR = parotid lymph node, POP = popliteal lymph node, MES = mesenteric lymph node, PLA = placenta, HLN = hepatic lymph node, TE = third eyelid, MAN = mandibular lymph node, TON = tonsil, ILN = ileocecocolic lymph node, RAM = recto-anal mucosa associated lymphoid tissue, SP = spleen, RLN = retropharyngeal lymph node, IL = ileum, TLN = tracheobronchial lymph node, SLN = sublumbar lymph node, OB = obex, MAM = mammary lymph node, CA = caruncle.</p
