Glioma cells seeded in a way that manifests cell crowding and cell dispersion show that at the core cells were more proliferative than glioma cells located at the rim.
<p>(A) SNB19 cells at the core of the cell circle stained for DAPI to account for all cells. (B) Same image field as panel A at the core of the cell circle but stained for Cyclin A (Cy3-red), (C) incorporated BrdU (FITC-green), and (D) showing CyclinA (Cy3-Red) - BrdU (FITC-green) Overlay. (E) SNB19 cells at the rim of the cell circle stained for DAPI to account for all cells. (F) Same image field as panel E but stained for Cyclin A (Cy3-red), (G) incorporated BrdU (FITC-green), and (H) showing CyclinA (Cy3-Red) - BrdU (FITC-green) Overlay. (I) and (J) Quantitative analysis of Cyclin A- and BrdU-labeled SNB19 and T98G glioma cell numbers (n = 3). The percent of Cyclin A-, and BrdU-labeled cells, and the total number of cells were measured. Statistical analysis was performed using a student T-test. * p<0.05.</p
