<p>(<b>a</b>) The representative western blots showing the expression of EGFR, ERK, and p-ERK before and after 10 ng/mL EGF treatment from both apical and basolateral sides in both cell types for the indicated time. (<b>b</b>) Summary of EGFR expression levels, all data points were normalized to the value obtained from cilia (-) cells under control condition, i.e. no EGF simulation (n = 5 for each data point). (<b>c</b>) Summary of p-ERK expression levels, all data points were normalized to the value obtained from cilia (-) cells under control condition, i.e. no EGF simulation (n = 6 for each data point). * indicates p-ERK expression level of cilia (-) cells is significantly greater than that of cilia (+) cells. (<b>d</b> & <b>e</b>) The representative western blots showing the expression ERK and p-ERK with and without stimulation by 10 ng/mL EGF from both apical and basolateral sides in both cell types for 10 min, in the presence of variety of inhibitors. Where Crtl indicates control and AG, GF, GO, PD, and SB represent AG1478, GF109203, GO6976, PD98059, and SB202190, respectively. Cells were preincubated with EGF for 60 min, respectively. (<b>f</b> & <b>g</b>) Summary of p-ERK expression levels, all data points were normalized to the value obtained under control condition, i.e. no EGF simulation (n = 5 for each data point). * indicates the values were dramatically less than those of control and EGF stimulation. <sup>#</sup> indicates p-ERK expression level is significantly greater compared to control.</p
