Composition of CXCL10-mucin-GPI as an example for a novel class of GPI-anchored chemokine fusion proteins.

Abstract

<p>The mucin domain of CX3CL1 was combined with a GPI anchor and a CXCL10 chemokine head to generate a flexible tool for the modification of tumor micromilieus capable of selectively stimulating the recruitment of CXCR3⁺ leukocytes. The chemokine head directs the specificity towards CXCR3⁺ leukocytes, while the mucin domain assists in the recruitment process and lowers the requirement for other adhesion molecules. Inclusion of a GPI anchor allows the protein to integrate into the cell membranes of tumor, stromal and endothelial cells when applied exogenously, thus superseding the transfer of genetic material into the tumor.</p