<p>A. <i>Ex vivo</i> phagocytosis of fluorescent <i>S. aureus</i> incubated with 50% human whole blood and Efb (1 µM). Neutrophils were gated based on forward and side scatter properties. B. <i>In vivo</i> phagocytosis of fluorescent <i>S. aureus</i> by human neutrophils in the mouse peritoneum. Neutrophils were attracted to the peritoneal cavity using carrageenan (i.p.) and subsequently challenged with 10<sup>8</sup> heat-inactivated fluorescent <i>S. aureus</i> and Efb (1 µM) for 1 hour. The peritoneal lavage was collected and neutrophil phagocytosis was analyzed by flow cytometry. Neutrophils were gated based on Gr-1 expression. The mouse experiments were carried out three times. In each experiment, we used 3 mice per group and the cells of these 3 mice were pooled for phagocytosis analysis. C. Representative histograms of B. A,B are mean ± se of three independent experiments. *<i>P</i><0.05, **<i>P</i><0.005 for Efb versus buffer (two-tailed Student's <i>t</i>-test).</p
