Knockdown of MCL-1 and ectopic expression of phosphorylation-deficient BAD are sufficient to induce apoptosis in prostate cancer cells.

Abstract

<p>C42Luc cells were co-transfected with either MCL-1-specific shRNA or scrambled shRNA in combination with either the BAD2SA or WT-BAD construct. 48 hours post-transfection, apoptosis was analyzed by time-lapse microscopy. At least 100 cells were counted for each treatment. Error bars show standard deviations from the average of four randomly chosen fields. *, <i>p</i><0.05 **, <i>p</i><0.005 ***, <i>p</i><0.001.</p