<p>A. MapMan output was used to illustrate the significant transcriptional changes across the tissue in GA metabolism at 6 h after reorientation. The reported values are log transformed ratios of transcripts in the lower flank to that in the upper flank. Each transcript is indicated as up-regulated (red square; increase in the ratio of the lower to upper transcript values) or down-regulated (blue square; decrease in the ratio of the lower to upper transcript values). Squares arranged in rows and columns represent individual transcripts at a single time point in the process. An individual square in a given area has the same color as the guide bar in the same column. A filled circle indicates that no transcript was detected. B. qRT-PCR analysis of <i>OsGA20ox4</i> (Os05g34854), <i>OsGA2ox7</i> (Os01g11150) and <i>OsGA2ox9</i> (Os02g41954) expression after horizontal reorientation. Relative changes in transcript abundance after gravity stimulation compared to the vertical control at each time point were analyzed by qRT-PCR, and results were compared with the microarray data. Relative changes in transcript abundance of qRT-PCR were all determined using the non-reoriented control at the relevant time point as reference. 0.5 h down, 0.5 h up, 6 h down and 6 h up represent for lower flank at 0.5 h, upper flank at 0.5 h, lower flank at 6 h and upper flank at 6 h, respectively. The relative gene transcript abundance was calculated as the ratio between the control and gravistimulated samples at each time point. Values are means ± SD; n = 3; □ real-time PCR results; ▪ microarray data.</p
