MUC1 and 16 expression is inversely correlated with sensitivity to ADCC-mediated killing.

Abstract

<p>CFSE labeled Capan-1 cells stained for MUC1 or MUC16 expression and propidium iodide (PI) uptake after 4 hrs co-culture with Erbitux® and PBMCs as analyzed by flow cytometry. A) Gating strategy on CFSE positive Capan-1 cells and from that population the selection of high and low MUC16 expressing cells. The gating strategy is the same for the MUC1 stained cells. B) The % PI positive cells are quantified in the MUC1/16 high and low expressor populations within the CFSE positive Capan-1 WT or COSMC KO cell population. Experiments performed with six different PBMC donors. Data from one representative donor is shown.</p

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