UCA1 may be involved in apoptosis induced by Ets-2 knockdown in BLZ-211 cells.

Abstract

<p>(A) The induction of apoptosis following a 48-hour treatment of Ets-2 siRNA or scrambled control siRNAs in BLZ-211 cells was examined by using a fluorescence microscope. Cells were stained with Annexin V (Green) and PI (Red). Scale bar 25 µm. The images were representative of three independent experiments. (B) Apoptosis was quantified by flow cytometry (FCM). The FCM results were representative of three independent experiments. Cellular pro-apoptosis processes (upper right quadrant) were increased after Ets-2 knockdown in BLZ-211 cells (12.49±1.21% vs. 6.40±1.47%, *<i>P</i><0.05) while no significant change in BLS-211 cells (4.12±0.28% vs. 3.20±2.50%, <i>P</i>>0.05). NSC: non-specific control siRNA.</p

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