<p>(A) GAS5 expression was inhibited by specific siRNAs in RT4, and the cell apoptosis was analyzed by flow cytometer 48 h later. (B) RT4 cells were treated with GAS5-siRNA or CDK6-siRNA. Forty-eight hours later, the relative cell numbers in each cell cycle phase after propidium iodide staining were determined by FACS analysis. The data are from one of three independent experiments. The histograms were analyzed and the percentage of cells in each phase of the cell cycle is shown. The results are presented as mean ± SD for three experiments.</p
