<p>(A) RT4 cells were treated with GAS5-siRNA, and GAS5 expression level was assayed after 48 h by real-time PCR. (B) RT4 cells were treated with GAS5-siRNA, and at the indicated time points, the numbers of cells per well were measured by the absorbance (450 nm) of reduced WST-8. (C) RT4 cells were treated with pcDNA-GAS5, and GAS5 expression level was assayed by real-time PCR. (D) RT4 cells were transiently overexpressed with GAS5, and the numbers of cells per well were measured by the absorbance (450 nm) of reduced WST-8. The results show data from at least three independent experiments, expressed as the mean ± SD. *<i>P</i><<i>0.05</i>.</p
