Long-Lived Luminogenic Probe for Detection of RNA
in a Crude Solution of Living Bacterial Cells
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Abstract
A pre-type
sensitizer for a lanthanide complex on an oligonucleotide
was successfully converted to a perfect final structure in a target
DNA/RNA-templated reaction, without any chemical reagent or enzyme,
under neutral conditions. The final form of the lanthanide–oligonucleotide
provided a long-lived luminescence signal, appropriate for time-gated
luminescence analysis and signal amplification. Target DNA/RNA-assisted
time-gated luminescence analysis is a powerful tool for elimination
of autofluorescence and detection of target RNA in living bacterial
cells