Identification of the reaction product of recombinant WsTR-I with tropinone as substrate.

Abstract

<p>TLC: I, tropine standard; II, WsTR-I reaction mixture after catalytic reaction termination; III, NaBH₄ reduction products (tropine and pseudotropine) of tropinone; IV, tropinone standard. GC: A, authentic tropinone; B, authentic tropine; C, control (enzyme minus) assay mixture of WsTR-I; D, experimental (complete) assay mixture of WsTR-I assay; E, NaBH₄ aided reduction products (tropine and pseudotropine) of tropinone.</p