A higher affinity PI3P-binding probe, 4x-FYVE^Hrs, reveals a larger pool of intracellular PI3P in the absence of Vps34 compared to the conventional 2x-FYVE^Hrs probe.

Abstract

<p>Control and <i>Vps34</i> KO MEFs were transiently transfected with both RFP-2x-FYVE^Hrs and GFP-4x-FYVE^Hrs PI3P-binding constructs for 24hr, grown in normal media and fixed. Confocal microscopy analysis of RFP-2x-FYVE^Hrs (red) and GFP-4x-FYVE^Hrs (green) is shown. Scale bar: 10 µm.</p