Expression of GHS-R mRNA in GnRH-GFP neurons.

Abstract

<p>Expression of GHS-R mRNA in GnRH-GFP neurons was demonstrated by single cell real-time PCR. The amplification plot shows the expression of <i>Gnrh</i> (Ct: 20.4), <i>Kiss1r</i> (Ct: 27) and <i>Gapdh</i> (Ct: 22.4) genes. The regular amplification curve of <i>Ghsr</i> with Ct values of 29.6 proves the expression of GHS-R mRNA.</p