The effect of DMY treatment on Jak/STAT signaling pathway in LPS−stimulated macrophages.

Abstract

<p>Cells were pre-treated with 50 μM DMY for 1 h, then with LPS treatment for 2, 4, and 8 h. Western blot analysis of (A) Phospho- and total Jak2 and STAT1 levels, (B) Total STAT3, phospho-STAT3, and SOCS3 levels and the levels of STAT3 and phospho-STAT3 in nuclear and cytosolic fractions. PARP and α-tubulin were used as internal control of nuclear and cytosolic proteins, respectively.</p

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