Culture of CB-NK cells.

Abstract

<p>Unselected CB MNCs were cultured for 7 days in a GP500 bioreactor with IL-2 (100 IU/mL) and aAPCs at 2∶1 aAPC:MNC ratio. Cells were immunomagnetically CD3-depleted on Day 7 and re-cultured in same conditions for an additional 7 days. On day 7 cells were again CD3-depleted and subject to phenotypic and functional studies.</p