Changes of Detergent-Resistant
Plasma Membrane Proteins
in Oat and Rye during Cold Acclimation: Association with Differential
Freezing Tolerance
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Abstract
Cold acclimation
(CA) results in an increase in freezing tolerance
of plants, which is closely associated to functional changes of the
plasma membrane (PM). Although proteomic studies have revealed compositional
changes of the PM during CA, there has been no large-scale study of
how the microdomains in the PM, which contains specific lipids and
proteins, change during CA. Therefore, we conducted semiquantitative
shotgun proteomics using microdomain-enriched detergent-resistant
membrane (DRM) fractions extracted from low freezing-tolerant oat
and highly freezing-tolerant rye. We identified 740 and 809 DRM proteins
in oat and rye, respectively. Among the proteins identified, the abundances
of a variety of proteins, such as P-type ATPase and aquaporins, were
affected by CA in both oat and rye. Some CA-responsive proteins in
the DRM fractions, such as heat shock protein 70, changed differently
in oat and rye. In addition, changes in lipocalins and sugar transporters
in the DRM fractions were different from those found in total PM fraction
during CA. This is the first report to describe compositional changes
in the DRM during CA. The proteomic profiles obtained in the present
study hint at many possible microdomain functions associated with
CA and freezing tolerance