Nano-Anchors with Single Protein Capacity Produced
with STED Lithography
- Publication date
- Publisher
Abstract
Acrylate
nanoanchors of subdiffraction-limited diameter are written
with optical stimulated emission depletion (STED) lithography. After
incubation, 98% of all nanoanchors are loaded quickly with fluorescently
labeled antibodies. Controlling the size of the nanoanchors allows
for limiting the number of the antibodies. Direct stochastic optical
reconstruction microscopy (dSTORM) imaging, statistical distribution
of fluorescence, quantitative fluorescence readout, and single molecule
blinking consistently prove that 80% of the nanoanchors with a 65
nm diameter are carrying only one antibody each, which are functional
as confirmed with live erythrocytes