<p>Representative photomicrographs of Neuro-2a cells stably expressing EGFP-LC3 with additional expression of NHE1 or NHE5 (A). Scale bar = 50 µm. Representative images of immunoblot analysis for expression of p62 and LC3 in EGFP-LC3-expressing Neuro-2a cells overexpressing indicated constructs (B). Bar graphs in B show quantification data of LC3-II expression levels relative to the level in EGFP-LC3-expressing Neuro-2a cells with no additional treatment normalized to β-actin expression. Results shown are mean ± SEM. *P<0.01, **P<0.05 (Student's t- test). Note that induced levels of LC3-II by overexpression of NHE1 and NHE5 was further increased by E64d/PepA, suggesting that expression of NHE1 and NHE5 induces autophagy in B.</p
