Decreased proliferation and change in physical properties of mutant optic vesicle.

Abstract

<p>(A) Immunohistochemical analysis for the presence of BrdU-labelled cells in the prospective neural retina on a coronal section of an E10.5 control embryo (left panel) and an <i>Lhx2-Cre:β-catenin<sup>flox/flox</sup></i> embryo (right panel). The part of the prospective neural retina that was analysed for BrdU<sup>+</sup> cells in the control and mutant embryos are outlined. (B) Immunohistochemical analysis for the presence apoptotic cells as revealed by the presence of activated Casp-3<sup>+</sup> cells on coronal section of an E10.5 control embryo (left panel) and a <i>Lhx2-Cre:β-catenin<sup>flox/flox</sup></i> embryo (right panel). (C) Immunohistochemical analyses for the presence of phosphorylated myosin light chain 2 (pMLC2) on a coronal section of an E10.5 control embryo. (D) Immunohistochemical analyses for the presence of pMLC2 on a coronal section on an E10.5 <i>Lhx2-Cre:β-catenin<sup>flox/flox</sup></i> embryo. Insets in C and D are a magnification of the areas indicated by the squares. Scale bars: (A, B and C, D) 100 µm.</p

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