<p>(A–C) <i>In situ</i> hybridization analyses of the indicated genes on coronal sections of E9.25 somite stage 21–23 (ss21–23) control embryos (left panels) and <i>Lhx2-Cre:β-catenin<sup>flox/flox</sup></i> embryos (right panels). (D–I) <i>In situ</i> hybridization analyses of the indicated genes on coronal sections of E9.75 (ss26–28) control embryos (left panels) and <i>Lhx2-Cre:β-catenin<sup>flox/flox</sup></i> embryos (right panels). (J–O) <i>In situ</i> hybridization analyses (K, M–O) or immunohistochemical analyses (J, L) for gene expression of the indicated genes on coronal sections of E10.5 (ss32–34) control embryos (left panels) and <i>Lhx2-Cre:β-catenin<sup>flox/flox</sup></i> embryos (right panels). β-catenin protein is indicated by red labelling in J and L while Mitf protein is indicated by green labelling in L. Arrows indicate the area where <i>β-catenin</i> has been inactivated in A, D and J. Arrow heads indicate the area of Axin2 expression in E and K. Dorsal-ventral (D–V) orientation for all panels is indicated in A. Scale bars: (A–C and D–O) 100 µm.</p
