<p>(<b>A</b>) Number of proteins found to associate with SF (Strep-Flag)-tagged human ARL13B using TAP (tandem affinity chromatography) and SILAC (stable isotope labelling affinity chromatography) approaches in HEK293 cells. (<b>B</b>) ARL13B complexes possess IFT-B proteins. White number; average peptide count from 4 independent tandem-affinity purification experiments (details in <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1003977#pgen-1003977-t001" target="_blank">Table 1</a>). Blue number; average enrichment scores from 4 independent experiments employing a single purification/SILAC quantitative approach. TTC26 also known as DYF-13 in <i>C. elegans</i>, and TTC30A also known as IFT70 in <i>Chlamydomonas</i>. (<b>C</b>) Western blot from HEK293T cells transfected with Arl13b-Flag vector or Flag vector ‘mock’ control (Flag) showing that immunoprecipitated Flag-tagged ARL13B associates with IFT-B proteins (IFT88, IFT52) but not Inversin (INVS). (<b>D</b>) Dedicated yeast-two hybrid one-on-one analysis reveals direct interactions between human ARL13B and both IFT46 and IFT74. WL; minimal media lacking Trp and Leu. WLHA; minimal media lacking Trp, Leu, His and Ade.</p
