Identification of Disulfide Bond Formation between
MitoNEET and Glutamate Dehydrogenase 1
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Abstract
MitoNEET is a protein that was identified
as a drug target for
diabetes, but its cellular function as well as its role in diabetes
remains elusive. Protein pull-down experiments identified glutamate
dehydrogenase 1 (GDH1) as a potential binding partner. GDH1 is a key
metabolic enzyme with emerging roles in insulin regulation. MitoNEET
forms a covalent complex with GDH1 through disulfide bond formation
and acts as an activator. Proteomic analysis identified the specific
cysteine residues that participate in the disulfide bond. This is
the first report that effectively links mitoNEET to activation of
the insulin regulator GDH1