<p>(A) Flow cytometry and (B) confocal laser scanning microscopy analyses of membrane estrogen receptors in BMMs. Intact cells labeled with E<sub>2</sub>-BSA-FITC or BSA-FITC alone as a control. For GPR30 detection, intact cells were pretreated with anti-GPR30 followed by FITC-conjugated secondary antibody. (C) Western blotting analyses of GPR30 protein from the membrane (mem), cytoplasmic (cyt), or nuclear (nu) fractions of BMMs. The results were verified by at least three independent experiments. The bar indicates 10 µm.</p
