<p>A) LTR = long terminal repeat; RRE = rev response element; cPPT = central polypurine tract; EF1a = elongation factor 1-Alpha; EGFP = green fluorescent protein; I = IRES; M = myristoylation; F36V = FKBP12; F36V' = modified FKBP12; cRANK = cytoplasmic domain of RANK; WPRE = WHP posttranscriptional regulatory element. B) Western blot probed with an antibody for FKBP12 showing overexpression of the iRANK construct in RAW264.7+iRANK cells migrating around 70 kDa. Cells transduced just with the F36V' domains (RAW264.7+F2) show a band around 30 kDa. No bands appear in the untransduced RAW264.7 cells.</p
