Cell survival study.

Abstract

<p>RAW264.7 cells and RAW264.7+iRANK cells were treated with either RANKL (40 ng/ml) or AP20187 (50 nM) for 4 days to allow osteoclasts to form. The supplemented media was removed, and cells were cultured for additional 0, 3 or 5 days in the presence (A) or absence (B) of inducers, and the number of TRAP-positive multinucleated cells (MuNC) per well was counted and averaged over 4 wells. *p<0.05.</p