<p>A) Immunostaining for BrdU (red) and Ki67 (green) in the anterior SVZ of 10 week old WT and <i>Hmgb2−/−</i> mice. B) Collapsed image of confocal Z-stacks from brain sections of compound <i>Hmgb2+/+</i>NestinGFP+ and <i>Hmgb2−/−</i>NestinGFP+ transgenic mice stained for GFAP, a marker of SVZ NSCs. GFAP (red), Nestin (green), DAPI (blue). (C) High magnification orthogonal view of a single confocal plane from brain sections of compound transgenic mice stained with GFAP at the SVZ/RMS junction. D) Quantification of SVZ BrdU+ cells and cell density in (A), E) Ki67+ cells and cell density in (A), F) BrdU+/Ki67+ cells and cell density in (A). All figures are mean+/− SEM, *p≤0.05, **p≤0.005. n = 4 WT and n = 5 <i>Hmgb2−/−</i> per group. (G) Quantification of Nestin+ and GFAP+ staining in WT and <i>Hmgb2−/−</i> SVZ in (B). All figures are mean+/− SEM; the Kruskal-Wallis test was used. ***p = 0.0002. n = 4 WT and n = 5 <i>Hmgb2−/−</i> per group. (H) Reconstruction of z-stacks used to generate the image in panel B of GFAP staining in the SVZ of <i>Hmgb2+/+</i> (WT) and <i>Hmgb2−/−</i> mice. DAPI (blue), GFAP (red), and Nestin (green).</p
