<p>Serial dilutions were performed with gDNA from CVS in a background of 1000(pg) mentioned is the total amount of fetal gDNA. M = 50 bp marker, 1 = 1000 pg, 2 = 500 pg, 3 = 250 pg, 4 = 125 pg, 5 = 60 pg, 6 = 30 pg, 7 = 15 pg, 8 = 7 pg, 9 = positive control for bisulfite conversion, 10 = NTC for bisulfite conversion, 11 = negative control for bisulfite conversion (non-bisulfite converted fetal gDNA), 12 = positive control for <i>mRASSF1A</i>-PAP, 13 = NTC for <i>mRASSF1A</i>-PAP. A 110 bp product (arrow) is obtained in cases where <i>mRASSF1A</i> sequences could be detected using <i>mRASSF1A</i>-PAP.</p
