Predicted and confirmed sequences for PAP-primer design.

Abstract

<p>Sequences of the <i>RASSF1A</i> gene were analyzed after bisulfite sequencing of maternal gDNA and fetal gDNA derived from CVS. Differentially methylated regions of the BisB region predicted by MethPrimer <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0084051#pone.0084051-Li1" target="_blank">[31]</a> could be confirmed using bisulfite sequencing. Both forward (A, upper panel, underlined) and reverse PAP-primer (B, upper panel, underlined) are specific for fetal sequences (middle panels) after bisulfite conversion and both primers have several mismatches to the maternal sequences (lower panels). Mismatches between fetal specific PAP-primers and maternal sequences are indicated with an * for each primer.</p