Deciphering the Human Brain
Proteome: Characterization
of the Anterior Temporal Lobe and Corpus Callosum As Part of the Chromosome
15-centric Human Proteome Project
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Abstract
Defining
the proteomes encoded by each chromosome and characterizing
proteins related to human illnesses are among the goals of the Chromosome-centric
Human Proteome Project (C-HPP) and the Biology and Disease-driven
HPP. Following these objectives, we investigated the proteomes of
the human anterior temporal lobe (ATL) and corpus callosum (CC) collected
post-mortem from eight subjects. Using a label-free GeLC–MS/MS
approach, we identified 2454 proteins in the ATL and 1887 in the CC
through roughly 7500 and 5500 peptides, respectively. Considering
that the ATL is a gray-matter region while the CC is a white-matter
region, they presented proteomes specific to their functions. Besides,
38 proteins were found to be differentially expressed between the
two regions. Furthermore, the proteome data sets were classified according
to their chromosomal origin, and five proteins were evidenced at the
MS level for the first time. We identified 70 proteins of the chromosome
15 – one of them for the first time by MS – which were
submitted to an in silico pathway analysis. These revealed branch
point proteins associated with Prader–Willi and Angelman syndromes
and dyskeratosis congenita, which are chromosome-15-associated diseases.
Data presented here can be a useful for brain disorder studies as
well as for contributing to the C-HPP initiative. Our data are publicly
available as resource data to C-HPP participant groups at http://yoda.iq.ufrj.br/Daniel/chpp2013. Additionally, the mass spectrometry proteomics data have been deposited
to the ProteomeXchange with identifier PXD000547 for the corpus callosum
and PXD000548 for the anterior temporal lobe