<p>Western blot analysis of total cell extracts and EVs of two independent human primary mammosphere preparations cultured in the presence of ethanol (EtOH), estrogen (E2) or tamoxifen (TAM). Protein expression was analyzed by immunoblotting with antibodies against CD13, Flotillin-1 and CD81 proteins. The molecular mass (kDa) for each protein is indicated. Representative micrographs of mammospheres formed under the different conditions are also shown. <i>Bar, 50</i> µ<i>m</i>.</p
