<p>RT-QuIC was performed using two different batches, Batch #1 (A) and Batch #2 (B), of hamster rPrP. Both assays employed hamster rPrP at a concentration of 60 µg/well as the substrate and sCJD M/V brain homogenate (BH) at the indicated dilutions as the seed. (C) RT-QuIC was performed using hamster rPrP Batch #1 at a concentration of 60 µg/well as the substrate and sCJD M/M brain homogenate (BH) at indicated dilutions to seed conversion. Reactions contained minimal salt (5.5 mM NaCl).</p
