<p>Cultures were synchronized by α-factor, UV-irradiated with 150 J/m<sup>2</sup>, and released back to growth media. Proteins from whole cell extracts, prepared from 1 ml of cell culture collected at the indicated time points after UV treatment, were analyzed by Western blotting. Anti-HA antibody was used to detect HA-tagged Pol3 (A to E), Pol31 (F), or Pol32 (G). Cell cycle progression was monitored by Clb2 cyclin levels, and PGK served as a loading control. The level of Pol3 relative to PGK is shown at the bottom of each panel.</p
