uPAR expression controls cell migration toward EGF.

Abstract

<p>Stably-transfected uPAR-293 cells (<b>A, C, E</b>) or V-293 cells (<b>B, D, F</b>) were pre-incubated with nonimmune Ig (<b>-</b>) or anti-uPAR or anti-uPAR_84–95 polyclonal antibodies (<b>A and B</b>), with diluents (-) or P-25 or W (W Pep) peptides (<b>C and D</b>), with diluents (-) or inhibitors of Rho- or Rac1-dependent signaling pathways (<b>E and F, left panels</b>), with diluents (-) or inhibitors of PI3K or ERK-MAPKs (<b>E and F, right panels</b>). Cells were then plated in Boyden chambers and allowed to migrate toward 100 ng/ml EGF. Migrated cells were fixed, stained with hematoxylin, and counted; results are expressed as percentage of cells migrated towards EGF over the cells migrated without EGF; 100% values represent cell migration in the absence of chemoattractants. The values are the mean±SD of three experiments performed in triplicate. (*) p≤0.05, as determined by the Student's <i>t</i> test.</p