<p>All assays detected Aβ1-40 and 1-42 using ELISA (Wako) on conditioned media from the cell type indicated. Ratios were normalized against the first control line listed on each panel. Statistical significance was determined via Student's t-Test, error bars reflect SEM. Each n equals an individual cell line (averaged biological triplicates) in 1 independent experiment. <i>A</i>. Aβ42/Aβ40 ratio is increased in day 14 differentiated NPCs/early neurons. Control and <i>PSEN1</i> NPCs were generated from the core set of iPSC lines, and one of three independent experiments is shown. For control compared to PSEN1 NPCs (n = 4 for each genotype), p = 0.003. <i>B–C.</i> Aggregate data is shown from 3 independent fibroblast and 3 independent NPCs/early neuron experiments. N = 7 for each fibroblast genotype data point, and n = 12 for each NPC/early neuron genotype data point. <i>B</i>. Aβ 42/40 ratios are shown for both control and PSEN1 fibroblasts and NPCs. For control fibroblasts vs. <i>PSEN</i>1 fibroblast, p = 0.001; for control NPCs vs. <i>PSEN1</i> NPCs, p = 0.000005; for <i>PSEN1</i> fibroblasts vs. <i>PSEN1</i> NPCs, p = 0.036. <i>C</i>. Total Aβlevels (Aβ40 + Aβ42) are statistically similar between control and <i>PSEN1</i> fibroblasts and NPCs/early neurons. See also <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0084547#pone.0084547.s003" target="_blank">Figure S3</a>.</p
