<p><sup>1</sup>H-<sup>15</sup>N-TROSY spectra of unbound DARC 1–60 (black) overlaid on <sup>1</sup>H-<sup>15</sup>N-TROSY spectra of DARC 1–60 in the presence of excess unlabelled DBP-RII (red). Sequence assignments are shown for the unbound DARC <sup>1</sup>H-<sup>15</sup>N-TROSY spectra. Peaks still visible in the presence of DBP-RII (red) are at DARC 1–60's N- and C- termini. Residues that disappear in the presence of DBP-RII are in the center of DARC and delineate the binding region.</p
