Electrochemical Biosensing Platform Using Hydrogel
Prepared from Ferrocene Modified Amino Acid as Highly Efficient Immobilization
Matrix
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Abstract
To
increase the loading of glucose oxidase (GOx) and simplify glucose
biosensor fabrication, hydrogel prepared from ferrocene (Fc) modified
amino acid phenylalanine (Phe, F) was utilized for the incorporation
of GOx. The synthesized hydrogel displays good biocompatibility and
contains a significant number of Fc moieties, which can be considered
as an ideal matrix to immobilize enzymes for the preparation of mediator-based
biosensors. The hydrogel was studied by scanning electron microscopy,
which indicated that it was composed of nanofibers with a diameter
of around 50β100 nm and length extended to 1 mm. With the addition
of GOx into the hydrogel and by directly dropping the resulting biocomposite
onto the electrode surface, a glucose biosensor, that displays good
performance due to improved enzyme loading and efficient electron
transfer, can be simply constructed. The favorable network structure
and good biocompatibility of the hydrogel could effectively avoid
enzyme leakage and maintain the bioactivity of the enzymes, which
resulted in good stability of the biosensor. The biosensor was utilized
for the detection of glucose in blood samples with results comparable
to those obtained from the hospital. The hydrogel as a functional
component of an amperometric biosensor has implications for future
development of biosensors and for clinical applications