<p>NO-dependent vasorelaxation was measured in the presence of apamin (5 µM), TRAM-34 (1 µM) and indomethacin (10 µM), and EDHF-dependent vasorelaxation was examined in the presence of L-NAME (100 µM) plus indomethacin (10 µM). Maximal relaxation (R<sub>max</sub>) was calculated as the leptin-induced percent decrease in tension developed in response to phenylephrine. EC<sub>50</sub>– leptin concentration (ng/ml) which induced a half-maximal relaxation of PE-preconstricted segments. R<sub>max</sub> and EC<sub>50</sub> values were calculated for each individual vascular preparation and data presented in the table are mean ± SD from 6 animals per group.</p>*<p>p<0.05,</p>**<p>p<0.01,</p>***<p>p<0.001 vs. control group (ANOVA and Tukey test).</p
