<p>(<b>A</b>) HPAEC analysis of the core glycans generated from <i>T. gondii</i> PTG strain protein-free GPIs II, III, V and VI. TLC-purified D-[<sup>3</sup>H]-glucosamine labeled GPIs were dephosphorylated, deaminated and reduced. The resulting neutral glycans were analysed before (untreated) and after Jack bean α-mannosidase or hexosaminidase treatments. The elution positions of the co-injected glucose oligomer standards are indicated at the top of each profile (bold digits) and given as DU. (<b>B</b>) Predicted Man-Man-(Glc-GalNAc)Man-anhydromannitol core glycan structure derived from protein-free GPIs II and V (right panel), and predicted Man-Man-(GalNAc)Man-anhydromannitol core glycan structure derived from protein-free GPIs III and VI (left panel) of the PTG strain. (<b>C</b>) ES-MS spectra of the PI moieties released by deamination from purified protein-free GPIs from RH (upper panel) and PTG (lower panel) strains.</p
