New Fluoranthene FLUN-550 as a Fluorescent Probe for
Selective Staining and Quantification of Intracellular Lipid Droplets
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Abstract
A new
class of live cell permeant, nontoxic fluoranthene-based
fluorescent probe (FLUN-550) having a high Stokes shift in aqueous
medium has been discovered. It showed selective staining of lipid
droplets (LDs, dynamic cytoplasmic organelles) at a low concentration
without background noise in in vitro live cell imaging of 3T3-L1 preadipocytes,
J774 macrophages, MCF7 breast cancer cells, and single-celled, parasitic
protozoa <i>Leishmania donovani</i> promastigotes and in
vivo nonparasitic soil nematode <i>C. elegans</i>