Induction
of Myogenic Differentiation of Human Mesenchymal Stem Cells Cultured
on Notch Agonist (Jagged-1) Modified Biodegradable Scaffold Surface
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Abstract
Engineered scaffold surface provides
stem cells with vital cues that could determine the eventual fate
of stem cells. In this work, biodegradable poly(l-lactide-<i>co</i>-ε-caprolactone) (PLCL) scaffold conjugated with
Notch agonist-Jagged-1(JAG) peptide (2.1 kDa) was prepared to initiate
myogenic differentiation of human mesenchymal stem cells (hMSCs).
The scaffold surface was activated with oxygen plasma and acrylic
acid was engrafted via UV polymerization to form a surface bearing
carboxylic groups. JAG peptide was subsequently immobilized onto the
carboxylated scaffold surface. Surface chemistry and topography were
examined using attenuated total reflection Fourier transform infrared,
X-ray photoelectron spectroscopy, and atomic force microscopy. Quantitative
real time polymerase chain reaction analysis revealed activation of
the Notch pathway; furthermore, several specific markers associated
with myogenic but not osteogenic differentiation were shown to be
up-regulated in hMSCs cultured on the engineered surface. The pro-myocardial
effect of surface bound JAG peptide was further affirmed via immunodetection
of the distinct myocardial marker, cardiac troponin T. Collectively,
our results suggest that PLCL conjugated JAG peptide is a viable strategy
to enhance the functional potential of scaffolds to be used as a bioengineered
cardiac patch in myocardial infarction repair
