<p>(A, B) To test plate colony formation of MCF-7 and SK-BR-3 cells transfected with pcDNA3.1/NNMT, cells were placed in wells with media and incubated for 14 days before counting the number of colonies (foci>50 µm). The plate efficiency of MCF-7/NNMT-1 and MCF-7/NNMT-2 cells was higher than that of MCF-7/Vector group. The similar result was found in SK-BR-3 cell models (B). (C, D) The colony formation numbers of MCF-7/NNMT-1 and MCF-7/NNMT-2 cells foci >100 µm after 14 days were more numerous than that of MCF-7/Vector group (*<i>P</i><0.05). The similar result was found in SK-BR-3 cell models (D). Values are expressed as means ± SD of four independent experiments.</p
