Biosynthesis of sulfated saccharides N-glycosidically linked to the protein via glucose. Purification and identification of sulfated dolichyl monophosphoryl tetrasaccharides from halobacteria

Abstract

A novel type of N-glycosidic linkage, asparaginyl glucose, occurs in the cell surface glycoprotein of halobacteria (Wieland, F., Heitzer, R., and Schaefer, W. (1983) Proc. Natl. Acad. Sci. U.S.A. 80, 5470-5474). Sulfated oligosaccharides containing glucuronic acids are attached to the polypeptide chain via this linkage. Here we describe the isolation and chemical characterization of lipid-linked precursors of these saccharides, and these have the following new features. Rather than the bacterial undecaprenol, a C60-dolichol is the carrier lipid. The oligosaccharide is bound to this lipid via a monophosphate, rather than a pyrophosphate bridge. Sulfation of the saccharides is completed while they are linked to lipid and does not occur after transfer of the saccharides to protein