Strigolactone-Regulated Proteins
Revealed by iTRAQ-Based
Quantitative Proteomics in <i>Arabidopsis</i>
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Abstract
Strigolactones
(SLs) are a new class of plant hormones. In addition
to acting as a key inhibitor of shoot branching, SLs stimulate seed
germination of root parasitic plants and promote hyphal branching
and root colonization of symbiotic arbuscular mycorrhizal fungi. They
also regulate many other aspects of plant growth and development.
At the transcription level, SL-regulated genes have been reported.
However, nothing is known about the proteome regulated by this new
class of plant hormones. A quantitative proteomics approach using
an isobaric chemical labeling reagent, iTRAQ, to identify the proteome
regulated by SLs in <i>Arabidopsis</i> seedlings is presented.
It was found that SLs regulate the expression of about three dozen
proteins that have not been previously assigned to SL pathways. These
findings provide a new tool to investigate the molecular mechanism
of action of SLs