<p>U2OS CALCR cells were stimulated with sCT for 4, 24, 48, 72 or 96(A) or hCT for 4, 24, 48 or 72 hours (B) in a similar prolonged setup as demonstrated in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0092042#pone-0092042-g002" target="_blank">Figure 2</a>. In parallel, one set of cells was stimulated for 96h (sCT) or 72h (hCT), following which the culture medium was replaced with fresh medium with ligands and incubated for another 4, 24 or 48 hours. Ligand concentration sCT or hCT: 10 nM, 1 nM, 0.1 nM or vehicle. The vertical arrow indicates time of medium-ligand replacement.</p
