Difference in CT_(a)R-mediated β-arrestin recruitment by prolonged continuous hCT and sCT stimulation.

Abstract

<p>A) Data show β-arrestin recruitment as a function of ligand concentration. U2OS CALCR cells were stimulated with sCT or hCT at a dose range of 0.1 nM to 100 nM for a prolonged period of time, ranging from 1, 4, 24, 48 to 72 hours. Ligands and medium were only added at the experiment initiation. B) Single dose of 100 nM sCT, 100 nM hCT and Vehicle shown in (A) plotted as a function of time. Asterisk (*) indicate significant difference between AUC sCT and AUC hCT, p&lt;0.05 was considered to be significant. *  = p&lt;0.05, **  = p&lt;0.01, ***  = p&lt;0.001. Data are shown as mean ± SEM of 3 experiments performed with five replicates.</p