<p>Splenocytes were labeled with CFSE and cultured for 72(LPS or ConA). For each mouse, dividing lymphocytes were determined as in the three examples presented here. (<b>A</b>) Cells were labeled with fluorescent antibodies and analyzed by flow cytometry. They were sorted with FSC/SSC profiles, separating viable lymphocytes (black arrow) from dead cells and cellular debris. (<b>B</b>) Among the viable lymphocyte gate, each subpopulation was selected as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0092664#pone-0092664-g003" target="_blank">Figure 3</a> and assessed for fluorescent division peaks. Generation 0 (black arrow) and each of the following generations (pink area) were used by the software to calculate the number of dividing cells.</p
