<p>A) Rec apoE4 (2 ug) was incubated together with purified human thrombin (5 U) in presence and absence of direct thrombin inhibitor argotroban (AGTB) at indicated concentrations for 16 h at 37°C. Since AGTB was dissolved in DMSO, DMSO control was included. AGTB was very potent in inhibiting thrombin mediated degradation of apoE. B) Rec apoE4 (2 ug) was incubated together with purified cathepsin G (cath G) at indicated concentrations in absence and presence of 100 nM cathepsin G inhibitor (CGI) for 16 h. ApoE proteolysis was analyzed by immunoblotting. C) Rec apoE3 and apoE4 (2 ug) were incubated with conditioned hippocampal medium for 16 h at 37°C in presence and absence of thrombin inhibitor, AGTB and cathepsin G inhibitor, CGI at indicated concentrations. ApoE was analyzed by western immunoblotting. Full length apoE levels were quantified as above.</p
